The Quality of Work Life (QWL) plays a crucial role in employee satisfaction and organizational success, particularly in service-oriented sectors like banking. This study explores gender-based perceptions of QWL among employees in private sector banks in Thiruvananthapuram District, focusing on factors such as compensation, teamwork, career growth, job security, and overall work environment. A quantitative survey approach was employed, collecting data from 100 employees (50 male and 50 female) across Federal Bank, South Indian Bank, and ICICI Bank. The findings reveal that while most aspects of QWL showed no significant gender differences, a statistically significant variance (p = 0.035) was found in perceptions of equitable and competitive compensation, with male employees rating it higher than their female counterparts. This suggests potential differences in salary negotiation practices, financial expectations, or historical perceptions of the gender pay gap. However, no significant gender differences were observed in other QWL factors, such as bonuses, teamwork, career advancement, and job security. The study concludes that inclusive HR policies and standardized workplace practices contribute to gender-neutral perceptions of QWL, except in the area of compensation fairness, which requires further examination. Recommendations include greater salary transparency, negotiation training for women, and continued efforts to promote gender equity in banking workplaces. This research underscores the importance of fair compensation practices and ongoing HR initiatives to enhance workplace equity in private sector banks.