The present study focuses on the phytochemical screening and in vitro anthelmintic activity of Bryophyllum pinnatum leaves, a traditionally used medicinal plant from the Crassulaceae family. Standardization of crude plant extracts is critical for guaranteeing the quality and therapeutic efficacy of herbal formulations. In this study, aqueous, methanolic, and chloroform extracts of Bryophyllum pinnatum leaves were prepared and screened for phytochemical constituents. The in vitro anthelmintic activity was evaluated using adult Indian earthworms (Pheretima posthuma), which exhibit anatomical and physiological similarities with human intestinal helminths. Different concentrations (25, 50, and 100 mg/mL) of each extract were tested, and parameters such as the time to paralysis and death were recorded. The methanolic extract at 100 mg/mL demonstrated significant anthelmintic activity, comparable to that of the standard drug piperazine citrate. Distilled water was used as a control. The results suggest that Bryophyllum pinnatum leaves, particularly in methanolic form, possess notable anthelmintic potential and can serve as a promising alternative to conventional therapies.