This paper introduces an advanced V-shaped photonic crystal fiber (PCF)-based surface plasmon resonance (SPR) biosensor. Numerical analysis of the sensor is carried out using the finite element method (FEM) in COMSOL Multiphysics 6.2. This incorporation of a V-shaped geometry improves the coupling between the core-guided mode and the surface plasmon mode, leading to optimized wavelength sensitivity and minimized confinement loss. The proposed sensor design is capable of detecting the analytes within a refractive index range of 1.38 to 1.42, making it suitable for applications in biochemical and biological detection, including drug analysis, antigen-antibody interactions, and gas sensing.