Human Metapneumovirus (HMPV) is a significant respiratory pathogen affecting individuals across all age groups, with heightened severity in young children, older adults, and immunocompromised patients. The aim of the study is to evaluate the knowledge levels of staff nurses regarding HMPV. A descriptive cross-sectional survey was conducted on staff nurses working in medical, surgical wards, and intensive care units (MICU and SICU) at GGS Medical College and Hospital, Faridkot, Punjab, India. Data were collected in November 2024, involving a sample of 50 nurses, using a structured questionnaire. Findings revealed that 20% of staff nurses demonstrated poor knowledge, 50% of nurses had moderate knowledge, 30% of nurses showed good knowledge. While 60% of nurses were aware of HMPV, only 45% understood its transmission. The highest awareness was among MICU nurses (60%), reflecting a higher exposure to respiratory infections. This study highlights the necessity for continuous professional development among nursing staff concerning emerging infectious diseases like HMPV.