Melia dubia, also referred to as Malabar neem, is a potential multipurpose tree ideal for agroforestry to increase yield per unit of land. It has several medical, pharmacological, ethnomedicinal, and conventional qualities and uses, including antioxidant, anticancer, antibacterial, and antidiabetic actions, and is widely sought after in herbal medicine. The aim of this paper is to assess the biological characteristics of Melia dubia seeds  through phytochemical characterization. The study emphasizes preliminary phytochemical analysis, total alkaloid content, in vitro antioxidant activity, and antimicrobial activities. The ferric-reducing antioxidant power method and nitric oxide radical scavenging activity methods were employed to examine the extract's antioxidant activity. For determining its antimicrobial activity, disc diffusion was used. It can be concluded that the ethanolic extract of Melia dubia seeds contains alkaloids, and the seeds exhibit both antimicrobial and antioxidant properties.